Method for rapid strep test
kit (ELISA indirect method)
(1) Loading: Take the pre-coated enzyme-linked plate coated with recombinant syphilis-specific antigen. Each control was provided with a blank control well, a negative serum control well, a positive serum control well, and a sample well. Add 50 μl of the sample dilution to each well of the rapid strep test kit. Then, 50 μl of each of the negative serum control, the positive serum control, and the serum to be tested were sequentially added. After mixing, it was incubated at 37 ° C for 30 minutes.
(2) Washing the plate: At the end of the incubation, discard the liquid in the well of the rapid strep test kit. Wash the plate five times with the washing solution and buckle.
(3) Enzyme-labeled secondary antibody: 100 μl of anti-human IgG enzyme-labeled antibody was added (not added to the blank control well). Mix well and incubate at 37 ° C for 30 minutes.
(4) The plate was washed again according to the method of Step 2, and color development was carried out: 50 μl of each of the coloring solutions A and B was added to each well in this order. Gently mix and mix at 37 ° C for 10 minutes.
(5) After termination (addition of stop solution 50 μl), the reaction was read and the result was judged: the blank well was zeroed with a microplate reader. The light absorption value (OD value) of each well was measured at a wavelength of 450 nm. The result is determined based on the ratio of the measured OD value to the critical value. If the ratio is greater than 1, it is a positive reaction, indicating that the sample contains syphilis antibodies. If the ratio is less than 1, it is a negative reaction, indicating that the sample does not contain syphilis antibodies.